11 Differences Between Light Microscope And Electron Microscope

11 Differences Between Light Microscope And Electron Microscope

A is that instrument that is utilized for the application of magnifying diminutive objects that is otherwise challenging to see via bare eyes. The two most typical kinds of microscopes that are employed for examination purposes are light microscope and electron microscope. These two Semtech solutions are highly sought-after ones as even beginners can use them seamlessly. But this is not the case with transmission electron microscopes as these are very challenging to utilize. Let us now learn about the differences that lie between light and electron microscope.

Listing out Differences for Proper Understanding

In a light microscope, the light source is positioned beneath the sample that is quite thin for permitting light for passing through. In case this doesn’t happen, the object will seem dark. Focusing on the light takes place on the sample with the help of a condenser lens that leads to a focused light point on the surface of the specimen. By interacting with the sample, the white light is transformed by the molecules that selectively absorb particular kinds of light. This leads to contrast and colour information that creates the image. Lenses then alter the projected detail into an exaggerated one that people can then perceive with their eyes.

When it comes to an electron microscope, the electron beam takes the portion of the source of the light. The electrons carry the duality of wave particle in a vacuum as these perform like the photons that are making up the light. But electrons travel in smaller wavelengths as compared to visible light that permits them in revealing minute details but this is always not possible with that of light. Silicon drift detectors utilize electrostatic or electromagnetic lenses but not glass condenser lens. This helps in producing electric and magnetic fields that emphasize and guide the electrons. The specimen will still be placed over a particular focused point of the beam but needs to be thinner in comparison to the size of the electrons. Similar to the optical microscope, the sample atoms come in interaction with the electron beam that carries away compositional and contrast details. The normal human eyes do not have the capacity of perceiving electrons. Hence these cannot function as the detector as it won’t be adequate. This makes the utilization of SEM scanning electron microscope a must for converting the electronic signal into a greyscale image. Here contrast resembles differences that lie in sample density and thickness.


Electron microscope Light microscope
Employs electron beams for magnifying small objects Employs visible light for magnifying objects
This is an advanced tool for the purpose of providing high-resolution magnification Object low enlargement possible in comparison to its electron equivalent
This carries a high-resolution power of around 0.001 µ in comparison to an optical one This optical microscope carries a resolution of around 0.25 µm
Electron microscope employs tungsten filament for releasing electrons Light microscope never employs any filament
This will only display dead and dried organisms This will display both dead and living organisms
Preparing the specimen takes up a lot of time which is few days Preparing the specimens only needs a few hours
Maintaining the instrument is quite pricey This instrument does not demand high cost for the purpose of maintenance
Viewing images is possible through fluorescent screens Viewing the pictures is possible even without screen
The image that people can view is perceived in white and black The images appear as coloured
The instrument carries an enlargement power of 1000000x The instrument carries a magnification power of up to 500x and can move up to 1000x as well
Radiation risks are totally present with this instrument There are zero radiation risks with this instrument



Now that we know what light microscope and electron microscope is all about, we can see that most microscopes are crucial in their own ways. While light microscopes find their usage in colleges and schools for viewing easily viewable organisms, electron microscopes find their utilization in laboratories by scientists for studying organisms in a detailed manner. With Semtech solutions, it is even possible to get hands-on effective microscope preventive maintenance. However before putting any of the microscopes to use, it is very important to carefully understand the differences between the two. Electron microscope gives you the possibility for cooling for the purpose of releasing the heat via current. But when it comes to light microscope, this instrument does not carry a cooling process within the system. Also for the former one, utilization of a vacuum is paramount for operating smoothly. For the latter one, you do not need to use a vacuum. Hence, depending on your usage, budget you must cautiously use the microscope. Along with this, while making the purchase for your laboratory, school or factory; always choose a reliable vendor to get support whenever you face challenges with the products.